Success stories
Quicker change - conventional vs. convenient mutant generation
This success story was provided by Dr. B. Pohn & Dr. S. Feichtenhofer (Institute of Molecular Biotechnology, Graz University of Technology, Austria)
11 mutations and a deletion at one sweep
evolving mutant generation from conventional to convenient
To answer the question concerning the correlation between a structural feature of a wild-type enzyme and the activity towards a […]
High GC content gene synthesis
This success story was provided by the group of Prof. Dr. Bechthold (Pharmaceutical Biology, Albert-Ludwigs-University, Freiburg)
This synthetic gene was prepared by Mr Gene while other companies refused to synthesize the gene due to a very high GC content.
Order submitted: March 18th - gene finalized: April 17th
Length: 471 bp - GC content: 71 %
The pharmaceutical activity […]
some like it hot - others like more protein!
This success story was provided by Felix List (graduate student at Prof. R. Sterner’s Lab; Lehrstuhl Biochemie II, Prof. Sterner; Institute of Biophysics and Physical Biochemistry; University of Regensburg)
1:Expression profile and codon quality of wt sequence
2:Expression profile and codon quality of optimized sequence
3:Protein Standard
(Expression strain: BL21 (DE3) Rosetta2, pET24a)
Background:
Sulfolobus solfataricus, a hyperthermophilic archaeon of the […]
