E.coli codon usage optimization
How to optimize a gene for E.coli codon usage using mrgene.com’s online software tool.
The movie below explains how to optimize a gene for E.coli codon usage.
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Mr. Gene’s online tool automatically tries to eliminate DNA motifs known to hamper expression in E.coli (and other prokarya). Ribosomal entry sites, TATA-Boxes or -35 Boxes present within the open reading frame worsen protein expression in almost any case.
Toward the end the movie shows how the codon optimized synthetic gene can be ordered online.
Please note: prices are a matter of change!
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