E.coli codon usage optimization
How to optimize a gene for E.coli codon usage using mrgene.com’s online software tool.
The movie below explains how to optimize a gene for E.coli codon usage.
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Mr. Gene’s online tool automatically tries to eliminate DNA motifs known to hamper expression in E.coli (and other prokarya). Ribosomal entry sites, TATA-Boxes or -35 Boxes present within […]
some like it hot - others like more protein!
This success story was provided by Felix List (graduate student at Prof. R. Sterner’s Lab; Lehrstuhl Biochemie II, Prof. Sterner; Institute of Biophysics and Physical Biochemistry; University of Regensburg)
1:Expression profile and codon quality of wt sequence
2:Expression profile and codon quality of optimized sequence
3:Protein Standard
(Expression strain: BL21 (DE3) Rosetta2, pET24a)
Background:
Sulfolobus solfataricus, a hyperthermophilic archaeon of the […]
